Sex estimation - long bones
Sex estimation from twelve osteometric measurements taken on the humerus, radius, femur and tibia, using a machine-learning model (linear discriminant analysis) validated on three reference samples.
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Measurement definitions
Upper limb
| Bone | Code | Measurement |
|---|---|---|
| Humerus | humxln | Maximum length |
| humhhd | Maximum vertical diameter of the head | |
| hummwd | Minimum midshaft diameter | |
| Radius | radxln | Maximum length |
| radtvd | Transverse diameter at midshaft | |
| radapd | Antero-posterior diameter at midshaft |
Lower limb
| Bone | Code | Measurement |
|---|---|---|
| Femur | femxln | Maximum length |
| femebr | Epicondylar breadth | |
| femhhd | Maximum diameter of the head | |
| femmtv | Medio-lateral diameter at midshaft | |
| Tibia | tibxln | Lateral condyle to medial malleolus length |
| tibpeb | Maximum breadth of the proximal epiphysis |
Training collections
The model was trained on a combined database of ten skeletal collections. The performance figures shown after an estimate come from three groups evaluated separately: Milan and Pretoria (marked below), each evaluated individually, and "BDC" (marked below), which combines the eight other collections evaluated together.
| Group | Country | Period | Sex | Females | Males | Total | |
|---|---|---|---|---|---|---|---|
| BDC | Goldman | International | Antiquity - 21st century | Estimated | 543 | 985 | 1528 |
| Laudun | France | 5th - 13th century | Estimated | 54 | 113 | 167 | |
| La Ciotat (LC) | France | 16th - 19th century | Estimated | 105 | 109 | 214 | |
| Marseille Petite Crotte (MPC) | France | 18th - 20th century | Estimated | 57 | 47 | 104 | |
| Terry | United States | 20th century | Known | 207 | 247 | 454 | |
| Olivier | France | 20th century | Known | 40 | 62 | 102 | |
| Nice | France | 21st century | Known | 21 | 19 | 40 | |
| NMDID (imagerie CT) | United States (New Mexico) | 2010 - 2017 | Known | 50 | 50 | 100 | |
| validation | CAL Milan | Italy | 21st century | Known | 199 | 199 | 398 |
| Pretoria Bone Collection (PBC) | South Africa | 21st century | Known | 180 | 180 | 360 | |
| Total | 1456 | 2011 | 3467 | ||||
References
Knecht, S., Santos, F., Ardagna, Y., Alunni, V., Adalian, P., & Nogueira, L. (2023). Sex estimation from long bones: a machine learning approach. International Journal of Legal Medicine, 137(6), 1887-1895. https://doi.org/10.1007/s00414-023-03072-4
Knecht, S., Krüger, G., Liebenberg, L., Ardagna, Y., Perrin, M., Ouladsine, M., ... & Adalian, P. (2026). Case-specific accuracy in sex estimation from long bones in forensic anthropology: an "Accuracy x-Factors" approach. Forensic Science International, 112820. https://doi.org/10.1016/j.forsciint.2026.112820
Knecht, S., Morandini, P., Biehler-Gomez, L., Ardagna, Y., Perrin, M., Cattaneo, C., ... & Adalian, P. (2026). Interpretable machine learning for individualized sex estimation from long bones. International Journal of Legal Medicine, 140(2), 983-995. https://doi.org/10.1007/s00414-025-03635-7
Knecht, S., Morandini, P., Biehler-Gomez, L., Ouladsine, M., Roman, C., Cattaneo, C., & Adalian, P. (2026). Population-specific validation of long bone linear discriminant analysis against morphoscopic pelvic methods for sex estimation in contemporary Italian skeletal remains. Journal of Forensic Sciences, 71(3), 1388-1397. https://doi.org/10.1111/1556-4029.70293